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ELISA Standard

By Ah Reum Kim | Oct 05, 2016 09:20AM CEST

Hello.
I want to know that my ELISA Standard Result with 4 parameter graph.
But, I don't know How to Use....

Conc(ng/ml) MeanValue(OD)
0.065 0.012
0.195 0.046
0.586 0.173
2.344 0.404
9.375 0.643
37.5 0.825

Here is my ELISA Results...

If i want to draw 4/5-parameter parallel lines logistic regression,
Should I put Concentration in Explanatory variables(X)?

also Should I Put MeanResult(OD) in Dependent variables(Y)?

I look forward to hearing from you on this matter at your earliest convenience. Thank you.

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By Thomas | Oct 05, 2016 02:08PM CEST | XLSTAT Agent

Dear Kim,

The Dependent variable shall be the dose effect measurement, so in your case it seems it must be MeanResult(OD).
If “Concentration” represents an injected quantity (not a measured effect), then it shall be set as Explanatory variables(X).

You can also read the tutorial linked below about the 4 or 5-parameter logistic regression :
https://help.xlstat.com/customer/en/portal/articles/2062264-run-4-or-5-parameter-logistic-regression-in-excel?b_id=9283

regards,

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